Desmond Miles (born 13 March 1987) was a traveling hermit, and a descendant to various lines of ancestors who had sworn their allegiance to the Assassin Order, which included individuals such as Aquilus, Altaïr Ibn-La'Ahad, Ezio Auditore da Firenze, and Connor. By age 16, Desmond was tired of living in hiding and aspired to pursue his own dreams. Using the little bit of Assassin training he had obtained, he fled from the Assassin compound he grew up in and managed to travel from South Dakota to New York City, where he found a living as a bartender in a successful nightclub.

In September 2012, Desmond was kidnapped and incarcerated at Abstergo Industries, a Templar organization, and forced into a machine called the Animus to relive the memories of his ancestor, Altaïr Ibn-La'Ahad, in order to acquire a map detailing all "Pieces of Eden" spread across the Earth. Having been successful in this, Desmond escaped Abstergo with the help of Lucy Stillman, an undercover Assassin, and joined an Assassin cell in Italy consisting of Shaun Hastings and Rebecca Crane. Once again entering an Animus, Desmond relived the memories of Ezio Auditore in order to obtain his skills.

Eventually, with knowledge gained from Ezio's memories, Desmond and his Assassin cell obtained the location of Ezio's Apple of Eden located underneath the Colosseum. Though successful in obtaining the Apple, Desmond's body was taken over by Juno, a member of the First Civilization, and was forced to stab Lucy. Desmond entered a coma as a result, entering the Black Room - the "safe mode" - of the Animus. With help from the AI clone of his Abstergo predecessor Clay Kaczmarek, "Subject 16", Desmond was able to regain consciousness, and was by this time located in New York once again. After which he destroyed the Templar's satalites and sent the Templars into hiding again. Months later, Lucy was revived by the gods of Greece, to which they started a relationship.

Meeting Ezio in personEdit

After sending the Templars into hiding, strange lights in the sky sent Desmond into the Animus again seeing that after Ezio died, his spirit disappeared. Hours later, Ezio appeared at his apartment door with Cole, Lucy(Kuo), their children and Zeke. To which they made a fast friendship.